输入文件clinical.png

输入文件risk.png

#引用包
library(ComplexHeatmap)
riskFile="risk.txt"          #风险文件
cliFile="clinical.txt"       #临床数据文件
setwd("E:\\research")

#读取风险文件
risk=read.table(riskFile, header=T, sep="\t", check.names=F, row.names=1)
risk=risk[order(risk$riskScore),] 

#读取临床数据文件
cli=read.table(cliFile,sep="\t",header=T,check.names=F,row.names=1)

#合并数据
samSample=intersect(row.names(risk), row.names(cli))
risk=risk[samSample,"risk",drop=F]
cli=cli[samSample,,drop=F]
rt=cbind(risk, cli)

#临床相关性分析，得到显著性标记
sigVec=c("Risk")
for(clinical in colnames(rt[,2:ncol(rt)])){
    data=rt[c("risk", clinical)]
    colnames(data)=c("riskScore", "clinical")
    data=data[(data[,"clinical"]!="unknow"),]
    tableStat=table(data)
    stat=chisq.test(tableStat)
    pvalue=stat$p.value
    Sig=ifelse(pvalue<0.001,"***",ifelse(pvalue<0.01,"**",ifelse(pvalue<0.05,"*","")))
    sigVec=c(sigVec, paste0(clinical, Sig))
    #print(paste(clinical, pvalue, Sig, sep="\t"))
}
colnames(rt)=sigVec

#定义热图注释
#rt=rt[apply(rt,1,function(x)any(is.na(match('unknow',x)))),,drop=F]
bioCol=c("#0066FF","#FF9900","#FF0000","#ed1299", "#0dbc21", "#246b93", "#cc8e12", "#d561dd", "#c93f00", 
         "#ce2523", "#f7aa5d", "#9ed84e", "#39ba30", "#6ad157", "#373bbf", "#a1ce4c", "#ef3bb6", "#d66551",
         "#1a918f", "#7149af", "#ff66fc", "#2927c4", "#57e559" ,"#8e3af4" ,"#f9a270" ,"#22547f", "#db5e92",
         "#4aef7b", "#e86502",  "#99db27", "#e07233", "#8249aa","#cebb10", "#03827f", "#931635", "#ff523f",
         "#edd05e", "#6f25e8", "#0dbc21", "#167275", "#280f7a", "#6373ed", "#5b910f" ,"#7b34c1" ,"#0cf29a" ,"#d80fc1",
         "#dd27ce", "#07a301", "#ddd53e",  "#391c82", "#2baeb5","#925bea", "#09f9f5",  "#63ff4f")
colorList=list(Risk=c("low"="green", "high"="red"))
j=0
for(cli in colnames(rt[,2:ncol(rt)])){
    cliLength=length(levels(factor(rt[,cli])))
    cliCol=bioCol[(j+1):(j+cliLength)]
    j=j+cliLength
    names(cliCol)=levels(factor(rt[,cli]))
    cliCol["unknow"]="grey75"
    colorList[[cli]]=cliCol
}

#绘制热图
ha=HeatmapAnnotation(df=rt, col=colorList)
zero_row_mat=matrix(nrow=0, ncol=nrow(rt))
Hm=Heatmap(zero_row_mat, top_annotation=ha)

#输出热图
pdf(file="heatmap.pdf", width=7, height=5)
draw(Hm, merge_legend = TRUE, heatmap_legend_side = "bottom", annotation_legend_side = "bottom")
dev.off()