WGCNA
TERThigh 与TERTlow分组方法:
TERT high (TERThigh) and low (TERTlow) cancers To identify telomerase-associated molecular signatures, we first defined two cancer types based on TERT expression:1) patients with high expression of TERT(TERThigh) and 2) patients with low expression of TERT(TERTlow). We excluded ALT cancers because of their small sample size (~ 5% of all samples in TCGA) andunique genomic and transcriptomic features that may confound the analysis. Samples with 2 or more RNA-Seq quantified reads of TERT were defined as TERThigh, while those with less than 2 reads of TERT and without known ALT-related somatic alterations (ATRX or DAXX mutations, deletions or structural variants) were define as TERTlow.
miRNA统计分析方法:
DNAmethylation
fisher exact test