全基因组组装,注释与评估软件集锦(更新于2020.03.20)

1.Assembly

1.1质体基因组

1.1.1NOVOPlasty

program language:Perl

Reference:

   10.1093/nar/gkw955(Nucleic Acids Research ,2016)

   10.1093/nargab/lqz011(NAR Genomics and Bioinformatics,2020)

Manual:https://github.com/ndierckx/NOVOPlasty

Link:https://github.com/ndierckx/NOVOPlasty

1.1.2GetOrganelle

program language:Python

Reference:10.1101/256479(bioRxiv ,2019)

Manual:https://github.com/Kinggerm/GetOrganelle

Link:https://github.com/Kinggerm/GetOrganelle

1.2核基因组

1.2.1Velvet

program language:C

Algorithm:De Bruijn graph(DBG)

Reference:

   10.1101/gr.074492.107(Genome Research ,2008)

   10.1371/journal.pone.0008407(Plos One,2009)

Manual:

    https://www.ebi.ac.uk/~zerbino/velvet/velvet_poster.pdf

    https://www.ebi.ac.uk/~zerbino/velvet/Manual.pdf

    https://github.com/dzerbino/velvet/blob/master/Manual.pdf

Link:

    https://github.com/dzerbino/velvet

    https://www.ebi.ac.uk/~zerbino/velvet/

1.2.2HGAP

Reference:10.1038/nmeth.2474(Nature Methods ,2013)

Manual:

    https://github.com/PacificBiosciences/Bioinformatics-Training/wiki/HGAP-1.4

    https://github.com/PacificBiosciences/Bioinformatics-Training/wiki/HGAP

Link:https://github.com/PacificBiosciences/Bioinformatics-Training/wiki/HGAP-1.4

1.2.3Falcon

program language:Python

Reference:10.1038/nmeth.4035(Nature Methods,2016)

Manual:https://github.com/PacificBiosciences/FALCON/wiki/Manual

Link:https://github.com/PacificBiosciences/FALCON

1.2.4Miniasm

program language:C

Reference:10.1093/bioinformatics/btw152(Bioinformatics,2016)

Manual:https://github.com/lh3/miniasm/blob/master/README.md

Link:https://github.com/lh3/miniasm/

1.2.5CANU

program language:C++

Reference:10.1101/gr.215087.116(Genome Research,2017)

Manual:https://github.com/marbl/canu/blob/master/documentation/source/tutorial.rst

Link:https://github.com/marbl/canu

1.2.6Hinge

program language:C++

Reference:10.1101/gr.216465.116(Genome Research,2017)

Manual:https://github.com/HingeAssembler/HINGE

Link:https://github.com/HingeAssembler/HINGE

1.2.7racon

program language:C++

Reference:10.1101/gr.214270.116(Genome Research,2017)

Manual:https://github.com/isovic/racon

Link:https://github.com/isovic/racon

1.2.8MECAT

program language:C++

Reference: 10.1038/nmeth.4432(Nature Maethods,2017)

Manual:https://github.com/xiaochuanle/MECAT

Link:https://github.com/xiaochuanle/MECAT

1.2.9SMARTdenovo

program language:C

Reference:no publication informations

Manual:https://yiweiniu.github.io/blog/2018/04/Genome-Assembly-Pipeline-SMARTdenovo/

Link:https://github.com/ruanjue/smartdenovo

1.2.10SOAPdenovo2

program language:C

Reference:no publication informations

Manual:https://github.com/aquaskyline/SOAPdenovo2

Link:https://github.com/aquaskyline/SOAPdenovo2

1.2.11wtdbg2-MPI

program language:C

Reference:

   10.1101/530972(bioRxiv,2019)

   10.1038/s41592-019-0669-3(Nature Methods,2019)

Manual:https://github.com/ruanjue/wtdbg2/blob/master/README-ori.md

Link:https://github.com/qzwlecr/wtdbg2-MPI

2.Annotation

2.1质体基因组

2.1.1GeSeq

Reference: 10.1093/nar/gkx391(Nucleic Acids Research,2017)

Manual:

    https://chlorobox.mpimp-golm.mpg.de/gs_documentation.html

    http://blog.sciencenet.cn/blog-3406804-1192085.html

Link:https://chlorobox.mpimp-golm.mpg.de/geseq.html

2.1.2PGA(Plastid Genome Annotator)

program language:Perl

Reference: 10.1186/s13007-019-0435-7(Plant Methods,2019)

Manual:

    https://github.com/quxiaojian/PGA

    //www.greatytc.com/p/6ac8a9fad9c9

    http://blog.sciencenet.cn/blog-3406804-1198528.html

Link:https://github.com/quxiaojian/PGA

2.2核基因组

2.2.1RepeatMasker(for repeat sequence)

program language:Perl

Reference:Smit, AFA, Hubley, R & Green, P. RepeatMasker Open-4.0.2013-2015 <http://www.repeatmasker.org>.

Manual:http://www.repeatmasker.org/webrepeatmaskerhelp.html

Link:http://www.repeatmasker.org/RMDownload.html

2.2.2BRAKER(for gene structure)

program language:Perl

Reference:

   10.1186/1471-2105-7-62(BMC Bioinformatics,2006)

   10.1093/bioinformatics/btn013(Bioinformatics,2008)

   10.1093/bioinformatics/btv661(Bioinformatics,2016)

   10.1007/978-1-4939-9173-0_5,1962:65-95(Methods in Molecular Biology,2019)

Manual:https://github.com/Gaius-Augustus/BRAKER

Link:https://github.com/Gaius-Augustus/BRAKER

2.2.3Maker (for gene structure)

Reference:10.1101/gr.6743907(Genome Research,2008)

Manual:

    https://weatherby.genetics.utah.edu/MAKER/wiki/index.php/Main_Page

    http://gmod.org/wiki/MAKER_Tutorial

Link:http://www.yandell-lab.org/software/maker.html

2.2.4MAKER-P  (for gene structure)

Reference:no pubilished info

Manual:https://weatherby.genetics.utah.edu/MAKER/wiki/index.php/Main_Page

Link:http://www.yandell-lab.org/software/maker-p.html

2.2.5eggNOG-mapper(for gene function)

program language:Python

Reference:

    10.1093/molbev/msx148(Molecular Biology and Evolution,2017)

    10.1093/nar/gky1085(Nucleic Acids Research,2019)

Manual:https://github.com/eggnogdb/eggnog-mapper/wiki/eggNOG-mapper-v2

Link:

   http://eggnog-mapper.embl.de/

   https://github.com/eggnogdb/eggnog-mapper

3.Correction

3.1pilon

program language:Scala

Reference:10.1371/journal.pone.0112963(PLoS ONE,2014)

Manual:https://github.com/broadinstitute/pilon/wiki/Methods-of-Operation

Link:https://github.com/broadinstitute/pilon

3.2redundans

program language:Perl

Reference:10.1093/nar/gkw294(Nucleic Acids Research,2016)

Manual:

     https://github.com/lpryszcz/redundans/blob/master/docs/poster.pdf

     https://github.com/lpryszcz/redundans

Link:https://github.com/lpryszcz/redundans

3.3TGS-GapCloser

program language:C++

Reference:10.1101/831248(bioRxiv,2019)

Manual:https://github.com/BGI-Qingdao/TGS-GapCloser

Link:https://github.com/BGI-Qingdao/TGS-GapCloser

4.Evaluation

BUSCO

Reference:10.1007/978-1-4939-9173-0_14(Methods in Molecular Biology,2019)

Manual:https://busco.ezlab.org/busco_userguide.html

Link:https://github.com/c-omics/busco

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