肺-GGN-ADC与ADC-markers

结果一

主要分群：
namely cancer cells (EPCAM; clusters 2, 5, 10, and 11), fifibroblasts (COL1A1; cluster 7), endothelial cells (CLDN5; cluster 9), T cells (CD3D; clusters 0 and 3), B cells (CD79A; cluster 12), NK cells (NKG7; cluster 4), mast cells (MS4A2; cluster 8), and myeloid cells (LYZ; clusters 1 and 6).

癌细胞鉴定：
We considered the cells marked by EPCAM were cancer cells referred to a scRNA-seq study of lung cancer（ref：8）

cancer cells (EPCAM; clusters 2, 5, 10, and 11)
fifibroblasts (COL1A1; cluster 7)
endothelial cells (CLDN5; cluster 9)
T cells (CD3D; clusters 0 and 3)
B cells (CD79A; cluster 12)
NK cells (NKG7; cluster 4)
mast cells (MS4A2; cluster 8)
myeloid cells (LYZ; clusters 1 and 6)

Snipaste_2021-01-22_13-31-28.png

Snipaste_2021-01-22_13-31-51.png

结果二

We identifified 50 different stromal cell subclusters (including 7 clusters from endothelial cells, 5 clusters from fifibroblasts, 17 clusters from T cells, 3 cluster from B cells, 4 cluster from NK cells, 11 clusters from macrophages, and 3 clusters from mast cells) and 13 cancer cell subclusters, of which, we focused on some important cell subclusters to explore their functions.

cancer cell subclusters

stromal cell subclusters：

7 clusters from endothelial cells
5 clusters from fifibroblasts
17 clusters from T cells
3 cluster from B cells
4 cluster from NK cells
11 clusters from macrophages
3 clusters from mast cells

结果三

Compared to normal cells at the bottom of each of the SADC and GGN-ADC groups in Fig. 2a (including endothelial cells, fifibroblasts, T cells, B cells, NK cells, myeloid cells, and mast cells), according to cell chromosome fragment variation, the cells marked by EPCAM were clustered into nine groups.